DNA evidence has become an increasingly important tool in Pakistan's criminal justice system, playing a pivotal role in solving complex cases. It involves analyzing genetic material to establish a connection between a suspect and a crime scene, helping to either confirm or eliminate their involvement. In Pakistan, the use of DNA evidence is particularly significant in cases of sexual assault, murder, and paternity disputes. DNA testing can provide accurate and reliable results, making it a key factor in court proceedings. However, its use is still developing, and there is a growing need for improved forensic facilities and training for law enforcement. The admissibility of DNA evidence is recognized under the legal framework in Pakistan, but it must be collected and handled properly to ensure its validity in court. As the technology continues to advance, DNA evidence is expected to play an even greater role in ensuring justice and reducing wrongful convictions in Pakistan's legal system.